Atg3^tm1Kmts
Targeted Allele Detail

Summary

• Symbol: Atg3^tm1Kmts
• Name: autophagy related 3; targeted mutation 1, Masaaki Komatsu
• MGI ID: MGI:5609520
• Gene: Atg3 Location: Chr16:44979192-45008901 bp, + strand Genetic Position: Chr16, 29.45 cM, cytoband B5
• Alliance: Atg3^tm1Kmts page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215884
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exon 10, which codes the active site cysteine. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:215884)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atg3 Mutation: 19 strains or lines available

References

• Original: J:215884 Sou YS, et al., The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice. Mol Biol Cell. 2008 Nov;19(11):4762-75
• All: 6 reference(s)