Ddiab32^LG/J
QTL Variant Detail

Summary

• QTL variant: Ddiab32^LG/J
• Name: diet based diabetes 32; LG/J
• MGI ID: MGI:5609688
• QTL: Ddiab32 Location: unknown Genetic Position: Chr11, Syntenic

Variant origin

• Strain of Specimen: LG/J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele affects insulin levels. (J:170533)

Notes

Trait Annotation
(using the Vertebrate Trait Ontology)

organism trait

metabolism trait

glucose metabolism trait - VT:0015088

altered insulin levels relative to mice expressing the SM/J allele - J:170533

Mapping and Phenotype information for this QTL, its variants and associated markers

J:170533

The mice used in this study were from the F16 generation of

the LG/J X SM/J Advanced Intercross Line (Wustl:LG,SM-G16).

The authors indicated 70 trait-specific QTL mapping to 64 locations,

and of these 70 a total of 17 are significant at the genome-wide level. Fifty three are significant at the chromosome level.

The 17 genome wide significant QTL are indicated with an '*' and can be viewed here on their respective chromosome mapping records (NCBI37/mm9).

The authors mentioned that phenotypic variation in response to glucosestresswas indicated by using an intraperitoneal injection of 0.01 ml of 10% glucose solution per

gram of body weight. The measurements taken over the course of 2 hours were used to calculate the area under the curve (AUC), which was used as overall measure of glucose tolerance.

Phenotypes were as follows:

AUC_10wks indicates under the curve at 10 weeks

AUC_20wks indicates area under the curve at 20 weeks

bGLC_10wks indicates basal glucose level at 10 weeks

bGLC_20wks indicates basal glucose level at 20 weeks,

GLC indicates serum glucose level at necropsy

INS indicates serum insulin level at necropsy

Chr MGI Author Pheno LOD Peak Mb 5 prime 3 Prime

11 *Ddiab31 Ddiab11a INS 4.98 7.32 6.46 7.85

11 *Ddiab32 Ddiab11b INS 8.15 12.52 11.51 13.77

11 Ddiab33 Ddiab11c AUC_20wks 2.88 80.69 80.01 81.6

11 Ddiab34 Ddiab11d AUC_20wks 3.15 105.13 104.43 106.39

11 Ddiab35 Ddiab11e AUC_20wks 3.03 108.38 107.61 109.26

Table 1 notes the presence of glucose and/or insulin affecting genes within the mapped QTL interval as markers Igfbp1 and Igfbp3 for QTL Ddiab31; and marker Grb10 within the interval of QTL Ddiab32.

References

• Original: J:170533 Lawson HA, et al., The importance of context to the genetic architecture of diabetes-related traits is revealed in a genome-wide scan of a LG/J x SM/J murine model. Mamm Genome. 2011 Apr;22(3-4):197-208
• All: 1 reference(s)