Igh^{tm3.1(IGH)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm3.1(IGH)Vlcg}
• Name: immunoglobulin heavy chain complex; targeted mutation 3.1, Velocigene
• MGI ID: MGI:5609988
• Synonyms: 80hVH, 80hVHdNeo
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208619
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Igh^{tm3.1(IGH)Vlcg} involves 2 genes/genome features (Adam6b, Adam6a) View all
• Igh^{tm3.1(IGH)Vlcg} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  human	IGH (3492)

• Mutation details: Using BAC recombineering roughly 3 Mbp of the mouse locus including all of the Vh Dh and Jh gene segments was replaced with 940 kb of contiguous human sequence containing the equivalent human gene segments. The proximal junction of the replacement was placed about 200 bp downstream from the last J segment and the distal junction was placed several hundred bp upstream of the most 5' V segment of the human gene and about 9 kbp downstream from J558.55. Flp-mediated recombination removed the selection cassette. (J:208619)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:208619 Macdonald LE, et al., Precise and in situ genetic humanization of 6 Mb of mouse immunoglobulin genes. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5147-52
• All: 3 reference(s)