Sox2^tm1.1Knd
Targeted Allele Detail

Summary

• Symbol: Sox2^tm1.1Knd
• Name: SRY (sex determining region Y)-box 2; targeted mutation 1.1, Hisato Kondoh
• MGI ID: MGI:5613017
• Synonyms: deltaN1, Sox2^deltaN1
• Gene: Sox2 Location: Chr3:34704554-34706610 bp, + strand Genetic Position: Chr3, 16.93 cM, cytoband A2-B
• Alliance: Sox2^tm1.1Knd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217059
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph, Modified regulatory region, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: By sequential targeting an IRES-EGFP and FRT flanked neo cassette were inserted downstream of the open reading frame. Flp-mediated recombination removed the neo cassette. Then a loxP site and neo cassette and loxP site were inserted around the N1 enhancer element. Cre-mediated recombination removed the N1 enhancer and neo cassette. Homozygous mice show loss of Sox2 expression in the zone at the caudal end of neural plate but expression is seen in the neural tube. (J:217059)

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox2 Mutation: 56 strains or lines available

References

• Original: J:217059 Takemoto T, et al., Tbx6-dependent Sox2 regulation determines neural or mesodermal fate in axial stem cells. Nature. 2011 Feb 17;470(7334):394-8
• All: 3 reference(s)