Tcf21^tm2.1Seq
Targeted Allele Detail

Summary

• Symbol: Tcf21^tm2.1Seq
• Name: transcription factor 21; targeted mutation 2.1, Susan E Quaggin
• MGI ID: MGI:5613240
• Gene: Tcf21 Location: Chr10:22693162-22696042 bp, - strand Genetic Position: Chr10, 10.33 cM
• Alliance: Tcf21^tm2.1Seq page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217144
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: One loxP site was inserted upstream of exon 1 and a second loxP site followed by an FRT flanked PGK-neomycin cassette was inserted in a nonconserved region of intron 1. The selection cassette was subsequently removed by expression of flp recombinase. (J:217144)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf21 Mutation: 16 strains or lines available

References

• Original: J:217144 Maezawa Y, et al., Loss of the podocyte-expressed transcription factor Tcf21/Pod1 results in podocyte differentiation defects and FSGS. J Am Soc Nephrol. 2014 Nov;25(11):2459-70
• All: 3 reference(s)