Angpt2^{tm1c(KOMP)Seq}
Targeted Allele Detail

Summary

• Symbol: Angpt2^{tm1c(KOMP)Seq}
• Name: angiopoietin 2; targeted mutation 1c, Susan E Quaggin
• MGI ID: MGI:5613889
• Gene: Angpt2 Location: Chr8:18740279-18791578 bp, - strand Genetic Position: Chr8, 10.3 cM
• Alliance: Angpt2^{tm1c(KOMP)Seq} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217296
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The Wtsi targeting vector PPGS00100_B_C02 was used for gene targeting. Exon 4 was flanked by loxP sites. In addition, an FRT site and a beta gal construct separated by a third loxP site from a neomycin sequance and an additional FRT site was inserted in intron 3. The beta gal and neomycin were subsequently removed by expression of flp recombinase leaving exon 4 flanked by loxP sites. (J:217296)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Angpt2 Mutation: 46 strains or lines available

References

• Original: J:217296 Thomson BR, et al., A lymphatic defect causes ocular hypertension and glaucoma in mice. J Clin Invest. 2014 Oct 1;124(10):4320-4
• All: 5 reference(s)