Rb1^tm2.1Fad
Targeted Allele Detail

Summary

• Symbol: Rb1^tm2.1Fad
• Name: RB transcriptional corepressor 1; targeted mutation 2.1, Frederick A Dick
• MGI ID: MGI:5614046
• Synonyms: Rb1^deltaG
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm2.1Fad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215358
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting construct introduced nucleotide substitutions in exons 15 and 17 that result in the amino acid substitutions of glutamic acid for arginine and lysine at positions 461 and 542, respectively (R461E, K542E). These mutations disrupt interaction with the transactivation domain of E2f1. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted upstream of exon 17. (J:215358)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 111 strains or lines available

References

• Original: J:215358 Cecchini MJ, et al., A retinoblastoma allele that is mutated at its common E2F interaction site inhibits cell proliferation in gene-targeted mice. Mol Cell Biol. 2014 Jun;34(11):2029-45
• All: 4 reference(s)