Rad50^tm5Jpt
Targeted Allele Detail

Summary

• Symbol: Rad50^tm5Jpt
• Name: RAD50 double strand break repair protein; targeted mutation 5, John H J Petrini
• MGI ID: MGI:5614071
• Synonyms: Rad50^Neo47
• Gene: Rad50 Location: Chr11:53540346-53598146 bp, - strand Genetic Position: Chr11, 31.98 cM, cytoband A5-B1
• Alliance: Rad50^tm5Jpt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209141
• Parent Cell Line: AC1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Nucleotide substitutions were introduced in exon 13, resulting in an amino acid substitution (p.V683R) in the Hook domain of the encoded peptide. A loxP site flanked neomycin resistance gene cassette was inserted into intron 13. The amino acid substitution introduces a charged residue in the vicinity of the cysteines that coordinate zinc and resulted in a hypomorphic allele. (J:209141)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad50 Mutation: 53 strains or lines available

References

• Original: J:209141 Roset R, et al., The Rad50 hook domain regulates DNA damage signaling and tumorigenesis. Genes Dev. 2014 Mar 1;28(5):451-62
• All: 1 reference(s)