Aars1^tm1Slac
Targeted Allele Detail

Summary

• Symbol: Aars1^tm1Slac
• Name: alanyl-tRNA synthetase 1; targeted mutation 1, Susan L Ackerman
• MGI ID: MGI:5614438
• Synonyms: Aars^stop
• Gene: Aars1 Location: Chr8:111759781-111784237 bp, + strand Genetic Position: Chr8, 57.9 cM
• Alliance: Aars1^tm1Slac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216802
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J/J

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: Using a modified BAC RP24-359N5 for homologous recombination, a C723 modification in exon 15 was made via site-directed mutagenesis using an FRT-flanked neo and arabinose-induced FLP, which left behind one FRT site, and a loxP-flanked Stop-neo cassette was additionally inserted into intron 1, which resulted in this constitutively expressed null allele that can be cre-excised to yield an expressed C723A mutant allele. (J:216802)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aars1 Mutation: 69 strains or lines available

References

• Original: J:216802 Liu Y, et al., Deficiencies in tRNA synthetase editing activity cause cardioproteinopathy. Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):17570-5
• All: 1 reference(s)