Pola2^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Pola2^{tm1b(KOMP)Mbp}
• Name: polymerase (DNA directed), alpha 2; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:5614599
• Gene: Pola2 Location: Chr19:5990570-6014230 bp, - strand Genetic Position: Chr19, 4.34 cM
• Alliance: Pola2^{tm1b(KOMP)Mbp} page
• IMPC: Pola2 gene page

Mutation origin

• Mutant Cell Line: DEPD00539_8_A08
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 6000931 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 6004368. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding a reporter knockout allele.Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157064, J:188991)

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pola2 Mutation: 35 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 4 reference(s)