Thratm1b(EUCOMM)Wtsi
Targeted Allele Detail
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Symbol: |
Thratm1b(EUCOMM)Wtsi |
Name: |
thyroid hormone receptor alpha; targeted mutation 1b, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5614615 |
Gene: |
Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
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Alliance: |
Thratm1b(EUCOMM)Wtsi page
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IMPC: |
Thra gene page |
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Mutant Cell Lines: |
EPD0204_5_A03, EPD0204_5_F02 |
Germline Transmission: |
Earliest citation of germline transmission:
J:188991
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Parent Cell Line: |
JM8.F6 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 98646667 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 98647289. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:155845, J:173534, J:188991)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
6 reference(s) |
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