Hpse^tm1.2Mdh
Targeted Allele Detail

Summary

• Symbol: Hpse^tm1.2Mdh
• Name: heparanase; targeted mutation 1.2, Mark D Hulett
• MGI ID: MGI:5615466
• Gene: Hpse Location: Chr5:100827350-100867582 bp, - strand Genetic Position: Chr5, 48.51 cM
• Alliance: Hpse^tm1.2Mdh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209913
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector inserted loxP sites flanking exon 1 and an adjacent neomycin resistance gene with another loxP site at the 3' end. The floxed neo and exon 1 were removed via cre-mediated recombination. RT-PCR and Western blot analysis confirmed absence of mRNA and protein. (J:209913)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hpse Mutation: 37 strains or lines available

References

• Original: J:209913 Poon IK, et al., Mice deficient in heparanase exhibit impaired dendritic cell migration and reduced airway inflammation. Eur J Immunol. 2014 Apr;44(4):1016-30
• All: 3 reference(s)