Myd88^tm1.1Medz
Targeted Allele Detail

Summary

• Symbol: Myd88^tm1.1Medz
• Name: myeloid differentiation primary response gene 88; targeted mutation 1.1, Ruslan Medzhitov
• MGI ID: MGI:5616077
• Synonyms: Myd88^FL
• Gene: Myd88 Location: Chr9:119165000-119169084 bp, - strand Genetic Position: Chr9, 71.33 cM
• Alliance: Myd88^tm1.1Medz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209373
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exons 3-5, encoding the Toll-IL-1 receptor (TIR) domain, were flanked by loxP sites. The frt-flanked neomycin resistance cassette was removed via flp-mediated recombination. When the floxed region is removed via cre-mediated recombination, splicing from exon 2 to exon 6 results in a frameshift mutation. (J:209373)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Myd88^tm1.1Medz/Myd88^tm1.1Medz Foxp3^tm4(YFP/icre)Ayr/Foxp3^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6
cn2	Myd88^tm1.1Medz/Myd88^tm1.1Medz Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * DBA/2

Phenotypes:

Affected Systems	cn1	cn2
show or hide all annotated terms
hematopoietic system		√
abnormal CD4-positive, alpha-beta T cell physiology		√
abnormal T-helper 1 physiology		√
abnormal T-helper 17 cell physiology		√
abnormal memory T cell physiology		√
immune system	N	√
immune system phenotype	N
abnormal CD4-positive, alpha-beta T cell physiology		√
abnormal T-helper 1 physiology		√
abnormal T-helper 17 cell physiology		√
abnormal memory T cell physiology		√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myd88 Mutation: 52 strains or lines available

References

• Original: J:209373 Schenten D, et al., Signaling through the adaptor molecule MyD88 in CD4+ T cells is required to overcome suppression by regulatory T cells. Immunity. 2014 Jan 16;40(1):78-90
• All: 5 reference(s)