Neb^tm2.1Chen
Targeted Allele Detail

Summary

• Symbol: Neb^tm2.1Chen
• Name: nebulin; targeted mutation 2.1, Ju Chen
• MGI ID: MGI:5618471
• Synonyms: NebdeltaSH3
• Gene: Neb Location: Chr2:52026652-52228810 bp, - strand Genetic Position: Chr2, 29.98 cM
• Alliance: Neb^tm2.1Chen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211217
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Insertion
• Mutation details: Residue I7097, encoded by the last exon of the gene, was replaced by an Frt-neo-Frt cassette and stop codons in all reading frames thereby eliminating the C-terminal SH3 domain. Flp mediated recombination removed the neo cassette. Western blot analysis confirmed the absence of the nebulin SH3 domain. (J:211217)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Neb Mutation: 401 strains or lines available

References

• Original: J:211217 Yamamoto DL, et al., The nebulin SH3 domain is dispensable for normal skeletal muscle structure but is required for effective active load bearing in mouse. J Cell Sci. 2013 Dec 1;126(Pt 23):5477-89
• All: 1 reference(s)