Hira^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hira^{tm1c(EUCOMM)Wtsi}
• Name: histone cell cycle regulator; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5619355
• Synonyms: Hira^flox
• Gene: Hira Location: Chr16:18695787-18789059 bp, + strand Genetic Position: Chr16, 11.69 cM, cytoband A-B1
• Alliance: Hira^{tm1c(EUCOMM)Wtsi} page
• IMPC: Hira gene page

Mutation origin

• Mutant Cell Line: EPD0181_1_A05
• Germline Transmission: Earliest citation of germline transmission: J:212533
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: Flp-mediated excison of the parental Hira^{tm1a(EUCOMM)Wtsi} allele resulted in the removal of the neomycin selection cassette and the inserted lacZ reporter sequence, leaving behind the loxP-flanked critical exon 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:173534, J:212533)

Expression

In Mice Carrying this Mutation:

3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hira Mutation: 74 strains or lines available

References

• Original: J:212533 Lin CJ, et al., Hira-mediated h3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote. Dev Cell. 2014 Aug 11;30(3):268-79
• All: 5 reference(s)