Tcf7l1^{tm1.1(cre/ERT2)Hng}
Targeted Allele Detail

Summary

• Symbol: Tcf7l1^{tm1.1(cre/ERT2)Hng}
• Name: transcription factor 7 like 1 (T cell specific, HMG box); targeted mutation 1.1, Hoang Nguyen
• MGI ID: MGI:5620299
• Synonyms: Tcf3^GC
• Gene: Tcf7l1 Location: Chr6:72603354-72766028 bp, - strand Genetic Position: Chr6, 32.27 cM
• Alliance: Tcf7l1^{tm1.1(cre/ERT2)Hng} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213957
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Tcf7l1^{tm1.1(cre/ERT2)Hng} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tcf7l1 (MGI:1202876)

• Mutation details: The targeting construct replaced the stop codon of exon 12 with an viral 2A self-processing peptide, EGFP reporter gene, a second viral 2A self-processing peptide, tamoxifen-inducible cre fusion gene (cre/ERT2) and FRT-flanked neomycin resistance cassette (2A-eGFP-2A-CreERT2-PGKneo). Flp-mediated recombination removed the selection cassette. (J:213957)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tcf7l1 (mouse) Summary of all recombinase alleles driven by Tcf7l1. Comparative matrix view of recombinase activities Alliance mouse Tcf7l1 gene page

Expression

In Mice Carrying this Mutation:

22 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf7l1 Mutation: 84 strains or lines available

References

• Original: J:213957 Howard JM, et al., Tcf3 expression marks both stem and progenitor cells in multiple epithelia. Development. 2014 Aug;141(16):3143-52
• All: 1 reference(s)