Lhx9^{tm1.1(cre/ERT2)Gan}
Targeted Allele Detail

Summary

• Symbol: Lhx9^{tm1.1(cre/ERT2)Gan}
• Name: LIM homeobox protein 9; targeted mutation 1.1, Lin Gan
• MGI ID: MGI:5620763
• Synonyms: Lhx9^GCE, Lhx9-GFPCreER^T2, Lhx9^{tm1.1(EGFP/cre/ERT2)Gan}
• Gene: Lhx9 Location: Chr1:138752924-138776315 bp, - strand Genetic Position: Chr1, 60.82 cM, cytoband G-H2
• Alliance: Lhx9^{tm1.1(cre/ERT2)Gan} page

Small body size of Lhx9^{tm1.1(cre/ERT2)Gan}/Lhx9^{tm1.1(cre/ERT2)Gan} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214080
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J x 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Lhx9^{tm1.1(cre/ERT2)Gan} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lhx9 (MGI:1316721)

• Mutation details: The targeting construct replaced exon 4 with an EGFP, inducible cre recombinase fusion gene (EGFP/cre/ERT2) and an FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining. Expression of the EGFP/cre/ERT2 fusion protein is detected by anti-estrogen receptor antibody staining in adult retinal neurons. (J:214080)

Generation of the Lhx9^{tm1.1(cre/ERT2)Gan} allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lhx9 (mouse) Summary of all recombinase alleles driven by Lhx9. Comparative matrix view of recombinase activities Alliance mouse Lhx9 gene page

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lhx9 Mutation: 31 strains or lines available

References

• Original: J:214080 Balasubramanian R, et al., Generation and characterization of Lhx9-GFPCreER(T2) knock-in mouse line. Genesis. 2014 Sep;52(9):827-32
• All: 2 reference(s)