Mapk4^tm1Melo
Targeted Allele Detail

Summary

• Symbol: Mapk4^tm1Melo
• Name: mitogen-activated protein kinase 4; targeted mutation 1, Sylvain Meloche
• MGI ID: MGI:5620956
• Synonyms: Erk4^-, Erk4^ki
• Gene: Mapk4 Location: Chr18:74061557-74198430 bp, - strand Genetic Position: Chr18, 50.31 cM
• Alliance: Mapk4^tm1Melo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219480
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The exon 2 coding sequence was replaced with an EGFP reporter gene and neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:219480)

Expression

In Mice Carrying this Mutation:

23 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mapk4 Mutation: 65 strains or lines available

References

• Original: J:219480 Rousseau J, et al., Targeted inactivation of Mapk4 in mice reveals specific nonredundant functions of Erk3/Erk4 subfamily mitogen-activated protein kinases. Mol Cell Biol. 2010 Dec;30(24):5752-63
• All: 3 reference(s)