Pip4k2c^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pip4k2c^{tm1b(KOMP)Wtsi}
• Name: phosphatidylinositol-5-phosphate 4-kinase, type II, gamma; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5629320
• Synonyms: Pip4k2c^-
• Gene: Pip4k2c Location: Chr10:127032936-127047454 bp, - strand Genetic Position: Chr10, 74.5 cM, cytoband D3
• Alliance: Pip4k2c^{tm1b(KOMP)Wtsi} page
• IMPC: Pip4k2c gene page

Immune infiltrates in the liver from Pip4k2c^{tm1b(KOMP)Wtsi}/Pip4k2c^{tm1b(KOMP)Wtsi} mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0383_6_B01, EPD0383_6_B02, EPD0383_6_D01
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P Mutation details: Cre-mediated excision of the parental Pip4k2c^tm1a(KOMP)Wtsi allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exons (exons 3 and 4), leaving behind the inserted lacZ reporter sequence. Western blot analysis of kidney tissue confirmed the absence of protein expression in homozygous mutant mice. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739, J:234283)

Generation of the Pip4k2c^tm1b(KOMP)Wtsi allele

Expression

In Mice Carrying this Mutation:	48 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pip4k2c Mutation: 32 strains or lines available

Notes

Cell lines EPD0383_6_B01 and EPD0383_6_B02 were successfully used to make chimeric mice. Germline transmission was accomplished. J:234283

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 6 reference(s)