Kcnq2^tm2.1Snhr
Targeted Allele Detail

Summary

• Symbol: Kcnq2^tm2.1Snhr
• Name: potassium voltage-gated channel, subfamily Q, member 2; targeted mutation 2.1, Shinichi Hirose
• MGI ID: MGI:5629428
• Synonyms: Kcnq2^Y284C
• Gene: Kcnq2 Location: Chr2:180717372-180777093 bp, - strand Genetic Position: Chr2, 103.57 cM, cytoband H3-4
• Alliance: Kcnq2^tm2.1Snhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213443
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

Allele Type: Targeted (Not Specified) Mutations: Insertion, Single point mutation Mutation details: RMCE using Kcnq2^tm1Snhr introduced a modified exon 6 in which a A to G point mutation results in the amino acid substitution of cysteine for tyrosine at position 284 (Y284C). Flp-mediated recombination removed the puromycin selection cassette. (J:213443)

Generation of the Kcnq2^tm1Snhr, Kcnq2^tm2.1Snhr, and Kcnq2^tm3.1Snhr alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnq2 Mutation: 50 strains or lines available

References

• Original: J:213443 Tomonoh Y, et al., The kick-in system: a novel rapid knock-in strategy. PLoS One. 2014;9(2):e88549
• All: 2 reference(s)