Capn8^{tm1(EGFP/cre/ERT2)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Capn8^{tm1(EGFP/cre/ERT2)Wtsi}
• Name: calpain 8; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5632390
• Synonyms: Capn8^{tm1(EGFP/cre/ERT2)Hmgu}
• Gene: Capn8 Location: Chr1:182392572-182459917 bp, + strand Genetic Position: Chr1, 85.37 cM, cytoband H4
• Alliance: Capn8^{tm1(EGFP/cre/ERT2)Wtsi} page

Mutation origin

• Mutant Cell Line: CEPD0089_2_C05
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Capn8^{tm1(EGFP/cre/ERT2)Wtsi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Capn8 (MGI:2181366)

• Mutation details: According to the allele molecular image presented by the International Knockout Mouse Consortium, this allele has loxP sites flanking exons 3 and 4, and inserted into the intron between exons 2 and 3 is one FRT site, an engrailed 2 splice acceptor sequence, an EGFP and cre/ERT2 expression cassette, and a Rox flanked puromycin resistance cassette. (J:155845)

Recombinase activity

Activity:	Tissue activity of this recombinase allele MGI has not yet included tissue activity data for this allele in any anatomical systems.
Driver:	Capn8 (mouse) Summary of all recombinase alleles driven by Capn8. Alliance mouse Capn8 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Capn8 Mutation: 40 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)