Prdm16^{tm1.1(EGFP/cre/ERT2)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prdm16^{tm1.1(EGFP/cre/ERT2)Wtsi}
• Name: PR domain containing 16; targeted mutation 1.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5633825
• Synonyms: Prdm16^{tm1.1(EGFP/cre/ERT2)Hmgu}
• Gene: Prdm16 Location: Chr4:154400582-154721330 bp, - strand Genetic Position: Chr4, 83.96 cM, cytoband E2
• Alliance: Prdm16^{tm1.1(EGFP/cre/ERT2)Wtsi} page

Mutation origin

• Mutant Cell Line: CEPD0008_9_E07
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Prdm16^{tm1.1(EGFP/cre/ERT2)Wtsi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prdm16 (MGI:1917923)

• Mutation details: According to the allele molecular image presented by the International Knockout Mouse Consortium, this allele has loxP sites flanking exon 2, and inserted into the intron between exons 1 and 2 is one FRT site, an engrailed 2 splice acceptor sequence, an EGFP and cre/ERT2 expression cassette, and a single Rox site remaining after a flanked puromycin resistance cassette removal. (J:155845)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prdm16 (mouse) Summary of all recombinase alleles driven by Prdm16. Alliance mouse Prdm16 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Prdm16 Mutation: 73 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)