Il1r1^tm1Quan
Targeted Allele Detail

Summary

• Symbol: Il1r1^tm1Quan
• Name: interleukin 1 receptor, type I; targeted mutation 1, Ning Quan
• MGI ID: MGI:5633953
• Synonyms: IL-1R1^r
• Gene: Il1r1 Location: Chr1:40264240-40356417 bp, + strand Genetic Position: Chr1, 18.81 cM
• Alliance: Il1r1^tm1Quan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219864
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The allele contains two knock-in sequences incorporated by two rounds of homologous recombineering. The floxed target I sequence is inserted between exon IX and exon X; it is composed of a loxP site, a mouse engrailed 2 splice acceptor sequence, a destabilization domain (DD), a 2xPolyA transcriptional STOP sequence, a neo cassette and a second loxP site. Target II sequence is inserted in place of the Il1r1 stop codon in exon XI; it is composed of 3 hemagglutinin tags (3xHA), a STOP codon fluorescent protein, an internal ribosome entry site (IRES), a tdTomato fluorescent protein sequence, a single FRT site and a STOP codon. Mice containing target I do not produce a functional protein. (J:219864)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il1r1 Mutation: 39 strains or lines available

References

• Original: J:219864 Liu X, et al., Interleukin 1 type 1 receptor restore: a genetic mouse model for studying interleukin 1 receptor-mediated effects in specific cell types. J Neurosci. 2015 Feb 18;35(7):2860-70
• All: 9 reference(s)