Sptbn1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sptbn1^{tm1c(EUCOMM)Wtsi}
• Name: spectrin beta, non-erythrocytic 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5635107
• Synonyms: Spnb2^co
• Gene: Sptbn1 Location: Chr11:30049395-30218175 bp, - strand Genetic Position: Chr11, 17.44 cM
• Alliance: Sptbn1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Sptbn1 gene page

Mutation origin

• Mutant Cell Line: EPD0065_1_F10
• Germline Transmission: Earliest citation of germline transmission: J:219548
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 30109028 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30109932. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:219548)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sptbn1 Mutation: 139 strains or lines available

References

• Original: J:219548 Lim JA, et al., Loss of beta2-spectrin prevents cardiomyocyte differentiation and heart development. Cardiovasc Res. 2014 Jan 1;101(1):39-47
• All: 1 reference(s)