Iqcg^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Iqcg^{tm1b(EUCOMM)Wtsi}
• Name: IQ motif containing G; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5635126
• Synonyms: Iqcg^KO
• Gene: Iqcg Location: Chr16:32834640-32876617 bp, - strand Genetic Position: Chr16, 23.55 cM
• Alliance: Iqcg^{tm1b(EUCOMM)Wtsi} page
• IMPC: Iqcg gene page

Mutation origin

• Mutant Cell Line: EPD0105_3_F01
• Germline Transmission: Earliest citation of germline transmission: J:218515
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 32855621 of Chromosome 16 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 32856630. The critical exon 6 is thus flanked by loxP sites. Cre expression results in a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:218515)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Iqcg Mutation: 32 strains or lines available

References

• Original: J:218515 Harris TP, et al., IQ motif-containing G (Iqcg) is required for mouse spermiogenesis. G3 (Bethesda). 2014 Feb;4(2):367-72
• All: 1 reference(s)