Tg(HTT*)AXwy
Transgene Detail

Summary

• Symbol: Tg(HTT*)AXwy
• Name: transgene insertion A, X William Yang
• MGI ID: MGI:5635157
• Transgene: Tg(HTT*)AXwy Location: unknown
• Alliance: Tg(HTT*)AXwy page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(HTT*)AXwy expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HTT (3064)

  Htt	(MGI:96067)

  Expresses a BAC with the first 17 amino acids deleted and exon 1 recplaced with 97 CAA-CAG repeats

• Mutation details: The 240 kb human bacterial artificial chromosome (BAC RP11-866L6), containing the entire 170 kb human Huntingtin genomic locus and approximately 20 kbp of 5' and 50 kbp of 3' flanking sequences and modified to replace the human HTT exon 1 with a loxP-flanked human mutant HTT exon 1 sequence containing 97 mixed CAA-CAG repeats encoding a continuous polyglutamine stretch was further modified to delete the first 17-amino-acid domain (N17; residues 2-16). This modified BAC containing both the 97Q repeats and the deleted N17 domain was microinjected into FVB/NJ fertilized eggs. Three founders were established; N, L, and A. Line A is the very low expressing line. (J:219890)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:219890 Gu X, et al., N17 Modifies mutant Huntingtin nuclear pathogenesis and severity of disease in HD BAC transgenic mice. Neuron. 2015 Feb 18;85(4):726-41
• All: 1 reference(s)