Nsun5^{tm2b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nsun5^{tm2b(EUCOMM)Wtsi}
• Name: NOL1/NOP2/Sun domain family, member 5; targeted mutation 2b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5635257
• Gene: Nsun5 Location: Chr5:135398807-135405659 bp, + strand Genetic Position: Chr5, 75.16 cM
• Alliance: Nsun5^{tm2b(EUCOMM)Wtsi} page
• IMPC: Nsun5 gene page

Mutation origin

• Mutant Cell Line: EPD0174_1_G02
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_gt0 cassette was inserted at position 135399453 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site was inserted downstream of the targeted exon(s) at position 135400680. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding this knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:155845, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:	50 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nsun5 Mutation: 30 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 5 reference(s)