Pold2^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Pold2^{tm1.1(KOMP)Vlcg}
• Name: polymerase (DNA directed), delta 2, regulatory subunit; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5635267
• Synonyms: Pold2^-
• Gene: Pold2 Location: Chr11:5822180-5828256 bp, - strand Genetic Position: Chr11, 3.88 cM, cytoband A2
• Alliance: Pold2^{tm1.1(KOMP)Vlcg} page
• IMPC: Pold2 gene page

Pold2^{tm1.1(KOMP)Vlcg}/Pold2^{tm1.1(KOMP)Vlcg} mice exhibit embryonic lethality, with normal number of embryos at E3.5 as blastocysts, but no embryos at E7.5. Blastocysts grown in vitro fail to hatch from the zona pellucida with apparent cell death during outgrowth culture.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: 13729A-H11
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 4825bp between positions 51793979-51798803 of Chromosome 7 (Genome Build37) and subsequent Cre-mediated excision deleted the neomycin selection cassette. (J:136110, J:188991)

Expression

In Mice Carrying this Mutation:

63 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pold2 Mutation: 31 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 6 reference(s)