Spag17^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Spag17^{tm1b(KOMP)Wtsi}
• Name: sperm associated antigen 17; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5635302
• Gene: Spag17 Location: Chr3:99792722-100050638 bp, + strand Genetic Position: Chr3, 43.25 cM, cytoband F3
• Alliance: Spag17^{tm1b(KOMP)Wtsi} page
• IMPC: Spag17 gene page

Mutation origin

• Mutant Cell Line: EPD0234_6_C05
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 99889093 of Chromosome 3 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 5 at position 99889997. Exon 5 was thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding this knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:	17 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Spag17 Mutation: 108 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 8 reference(s)