Adcyap1^{tm1.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Adcyap1^{tm1.1(cre)Hze}
• Name: adenylate cyclase activating polypeptide 1; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5635438
• Synonyms: Adcyap1-2A-Cre, PACAP-2A-Cre
• Gene: Adcyap1 Location: Chr17:93506448-93513967 bp, + strand Genetic Position: Chr17, 60.67 cM
• Alliance: Adcyap1^{tm1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220523
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Adcyap1^{tm1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Adcyap1 (MGI:105094)

• Mutation details: The targeting vector contains from 5' to 3', a partial Adcyap1 sequence spanning intron 1 through part of exon 5 (with an FRT3 site in intron 4) up to but not including the endogenous stop codon, a viral 2A oligopeptide sequence (F2A; mediates ribosomal skipping), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK-Neo, an FRT5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Adcyap1 (mouse) Summary of all recombinase alleles driven by Adcyap1. Comparative matrix view of recombinase activities Alliance mouse Adcyap1 gene page

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adcyap1 Mutation: 22 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 19 reference(s)