Cia52^DBA/1J
QTL Variant Detail

Summary

• QTL variant: Cia52^DBA/1J
• Name: collagen induced arthritis QTL 52; DBA/1J
• MGI ID: MGI:5635562
• QTL: Cia52 Location: Chr14:73082381-73082509 bp Genetic Position: Chr14, Syntenic

Variant origin

• Strain of Specimen: DBA/1J

Variant description

• Allele Type: QTL

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:201946

Collagen-induced arthritis (CIA) is the most commonly used model of rheumatoid arthritis (RA) in mice. In this study a known locus, Cia6, and a genomic region of Chromosome 14 were dissected using a partial advanced intercross (PAI) and a collection of congenic strains.

The congenics used for the PAI [[B10.D1-(D6Mit86-D6Mit188) H2^q/Rhd, B10.D1-(D6Mit268-D6Mit65) H2^q/Rhd and B10.D1-(D14Mit212-D14Mit160) H2^q/Rhd] were produced as speed congenics by marker assisted backcrossing of the susceptible DBA/1 (DQ) mice to the resistant, recipient strain C57BL/10.Q for seven generations. Four hundred and eighty four male, mice were tested in arthritis experiments. Seven markers were either linked to arthritis in single locus scans or involved in significant interactions detected in a two-locus scan.

To confirm and further define the indicated locus regions subcongenic strains were produced by backcrossing selected DBA/1 fragments from the PAI to the B10.Q background. Two hundred and fifty six heterozygous, male mice and 273 heterozygous, female mice were tested for CIA susceptibility and antibody production. The peak markers and marker intervals found in the congenic mice correlated well with the loci found in the PAI. Three new loci were detected on Chromosome 6 and four new loci on Chromosome 14.

In the PAI, the Cia52 locus, at peak marker D14Mit115 on chromosome 14, was only detected in an interaction with Cia49 affecting IgG1 production (LOD 7.9).

In the congenic mice Cia52, peak also mapped at D14Mit115, affected the incidence (p=0.003) and the accumulated score of arthritis (p=0.005) as well as the production of all antibody isotypes measures in female mice (IgG1 p=0.0008, IgG2a p=0.02, IgG3 p=0.01). No significant differences were noted in male mice.

References

• Original: J:201946 Popovic M, et al., Identification of new loci controlling collagen-induced arthritis in mouse using a partial advanced intercross and congenic strains. Scand J Immunol. 2008 Oct;68(4):405-13
• All: 1 reference(s)