Lgr4^{tm1.1(cre/ERT2)Npa}
Targeted Allele Detail

Summary

• Symbol: Lgr4^{tm1.1(cre/ERT2)Npa}
• Name: leucine-rich repeat-containing G protein-coupled receptor 4; targeted mutation 1.1, Novartis Pharma AG
• MGI ID: MGI:5635820
• Synonyms: Lgr4ki, Lgr4-mCherry-IRES-CreERT2
• Gene: Lgr4 Location: Chr2:109747992-109844602 bp, + strand Genetic Position: Chr2, 56.65 cM
• Alliance: Lgr4^{tm1.1(cre/ERT2)Npa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212415
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Lgr4^{tm1.1(cre/ERT2)Npa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lgr4 (MGI:1891468)

• Mutation details: The targeting construct replaced exon 1 following the start codon and 287 bp of intron 1 with an mCherry reporter gene, IRES, inducible cre recombinase fusion (cre/ERT2), polyadenylation sequence and FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. Antibody testing and direct fluorescence failed to detect mCherry. In situ hybridization confirmed the absence of transcript expression in the intestin, skin and kidney. (J:212415)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr4 (mouse) Summary of all recombinase alleles driven by Lgr4. Comparative matrix view of recombinase activities Alliance mouse Lgr4 gene page

Expression

In Mice Carrying this Mutation:	82 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr4 Mutation: 94 strains or lines available

References

• Original: J:212415 Kinzel B, et al., Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice. Dev Biol. 2014 Jun 15;390(2):181-90
• All: 2 reference(s)