Gnf5^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Gnf5^C57BL/6J
• Name: growth and fatness 5; C57BL/6J
• MGI ID: MGI:5635850
• QTL: Gnf5 Location: Chr2:152699221-152699361 bp Genetic Position: Chr2, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:152067

To determine QTLs effecting body weight C57BL/6J mice were mated to PWK/PhJ and the resulting F2 hybrids were used in mapping. A total of 479 F2 mice (235 female and 244 male) were bred from two types of reciprocal crosses: 236 and 243 F2 mice were obtained from (B6 female X PWK male) F1 Female X (B6 female X PWK male) F1 male crosses and (PWK female X B6 male) F1 female with (B6 female X PWK male) F1 male crosses respectively.

Four phenotypes were analyzed, these were body weight, lean weight,fat weight, and percent fat (fat weight/body weight X 100). C57BL/6J mice are heavier then PWK/PhJ mice.

The following new and existing QTLs were mapped

(following the QTL is the linked peak marker with cM locations):

Fatq1(D1Mit440 - 54 cM),

Pbwg1.1(D2Mit285 - 86 cM),

Adip22 (was identified as Adip10)(D2Mit293 -11cM),

Gnf5 (D2Mit285 - 86 cM),

Wt10q4 (was identified as Wt10q2) (D3Mit57 - 55 cM),

Btwq13 (D3Mit5 - 25cM),

Afw13 (was identified as Afw1) (D3Mit5 - 25 cM),

Afpq8 (was identified as Afpq1)(D3Mit256 - 66cM),

Bw6b (D3Mit17 - 32 cM),

Epb4.1 (D4Mit17 - 32 cM),

Adip23 (was identified as Adip11)Adip11 (D4Mit17 -32 cM),

Bwq8 (D5Mit123- 3cM),

Bwtq14 (was identified as Bw6d and Bwob) (rs3719351 - 49 cM),

Afw3 (D5Mit314 - 59 cM),

Bwtq7 (was identified as Bwtq10)(D6Mit198 - 67 cM),

Pfat5 (D6Mit284 - 38 cM),

Pbwg3(D7Mit223 - 72 cM),

Mtbcq9 (was identified as Mtbcq8)(D9Mit129 - 26 cM),

Adip21 (was identified as Adip5)(D9Mit123 - 42 cM),

Adip24 (was identified as Adip15)(D10Mit96 - 56 cM),

Bw4(D11Mit285 -48 cM),

Adip19 (D11Mit285 - 52 cM),

Fatpad3 ( D13Mit144 - 48 cM),

Bw40 (was identified as Bw10) (D13Mit144 - 48 cM),

Fatq1 (D16Mit107 - 3 cM)'

and Adip25 (was identifed as Adip17) (D16Mit107 - 3 cM).

The new QTLs mapped in this study were:

Bwtq13,

Bwq13 (alias Bwq8),

Bwq9 (alias Pbwq1.1),

Bwq11 (alias Pbwq3),

Pfat5 (was identified as Pfat2),

Gnf5 (was identified as Gnf3)

and Adip19.

10.03.2014 Curator Note:

We had originally associated the abdominal fatweight QTL identified here mapping to (D3Mit5- 25 cM) on mouse Chr 3, as Afw1. Afw1 was originally mapped using an outbred cross of DU6 xDUK, in J:49578 in 1998. We consider the current mapping study a separate mapping experiment and have named the QTL identified here as Afw13.

01.07.2015 Curator Note:

We had originally associated the QTL identified here, mapping to D13Mit144 - 48 cM as Bw10. However, Bw10 was originally mapped using a different cross, an outbred cross of DU6 x DUK, in J:49578, in 1998. We consider the current mapping study aseparate mapping experiment and have named the QTL identified here as Bw40.

01.23.2015 Curator Note:

Because Adip5 was origniallymapped in J:62610 in 2001 using an (LG/J x SM/J) intercross, which differs from the cross used here, we consider the current study a separate mapping experiment and have named this QTL Adip21.

Adip10, Adip11, Adip15 and Adip17 mentioned here were all originally mapped in J:103841 in 2006 using an (SM/J x NZB/BlNJ)F2 intercross, which alsodiffers from the cross used here. For thesame reasons noted above we have named these markers Adip22, Adip23, Adip24 and Adip25 respectively.

03.11.2015 Curator Note:

Because Bwtq10 was originally mapped in J:103841 in 2006 using (SM/J x NZB/BlNJ)F2 intercross, which differs from thecross usedhere, we consider the current study a separate map experiment and have named this QTL Bwqt7, (D6Mit198- 67 cM).

04.2.2015 Curator Note:

Mtbcq8 was originally mapped in J:93070 in 2004 using an (NMRI8/Fbn x DBA/2OlaHsd)F2 intercross, which differs fromthe cross used here, we consider the current study a new map experiment and have named the QTL Mtbcq9 (D9Mit129 - 26cM).

04.14.2015 Curator Note:

Because the abdominal fat percent QTL 1, (Afpq1) was orginally mapped in J:49578 in 1998 using an an outbredcross of DU6 xDUK, which differs from the cross used here we consider the current mapping study a separate mapping experiment and have named the QTL Afpq8.

04.15.2015 Curator Note:

Because Wt10q2 was originally mapped in J:55483 in 1999 using an (MHxC57BL/6J)F2 intercross, which differs from the cross used here, we consider the current mapping study a separate mapping experiment and have named the QTL Wt10q4.

06.16.2015 Curator Note:

Pbwq1.1 was orginally mapped in J:121981 using B6.Cg congenic mice, which differ from the C57BL/6J X PWK/PhJ mice used here. We consider the current mapping study a separate mapping experiment and have named this QTL, mapping to Chr 2 with D2Mit285, Bwq9.

Pbwq3 was originally mapped in J:88536 using an (C57BL/6J x M. m. castaneus)F1 x M. m. castaneus backcross, which also differs from the cross used here. We have named this QTL, mapping to Chr 7 with D7Mit223, Bwq11.

References

• Original: J:152067 Shao H, et al., Genetic loci affecting body weight and fatness in a C57BL/6J x PWK/PhJ mouse intercross. Mamm Genome. 2007 Dec;18(12):839-51
• All: 1 reference(s)