Hdlq95^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Hdlq95^C57BL/6J
• Name: HDL QTL 95; C57BL/6J
• MGI ID: MGI:5636050
• QTL: Hdlq95 Location: unknown Genetic Position: Chr8, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: This allele confers increased plasma HDL cholesterol in females on a CHOW diet compared to 129S1/SvImJ. (J:144089)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:144089

Linkage analysis was performed on 528 animals from a (C57BL/6J x 129S1/SvImJ)F2 intercross to map QTL associated with plasma HDL levels on either a CHOW or atherogenic diet. F2 animals were fed a CHOW diet until 8 weeks of age and then placed on an atherogenic diet until 16 weeks of age. An array of 508 SNPs was used for genome scan. Plasma HDL is significantly higher in 129S1/SvImJ parental strain compared to C57BL/7J on CHOW and high fat diets, with a stronger effect observed in males compared to females. Several previously identified HDL QTL were detected in this study as well as several novel loci, some of which showed interactive effects.

On mouse Chromosome 8, a female specific locus named Hdlq44 mapped to 79.7 Mb (34 cM) near rs13479840 (LOD=4.3). This locus displays significant linkage to plasma HDL concentration on CHOW diet with C57BL/6J-derived alleles conferring increased plasma HDL in females. The 95% confidence interval for Hdlq44 spans 71 Mb (33 cM) to 90 Mb (43 cM).

12.15.2014_djr Because Hdlq44 was originally mapped in J:114073 in 2003 using an (C57BL/6J x A/J)F2 intercross, which differs from the cross used here, we consider the current study a separate mapping experiment and have named this QTL Hdlq95.

Hdlq16 (HDL QTL 16) mappeddistally at 107.7 Mb (51 cM) in linkage to plasma HDL concentration on a CHOW diet at rs30731821 (LOD=3.4). C57BL/6J-derived alleles at Hdlq16 confer increased plasma HDL values. The 95% confidence interval for Hdlq16 spans 94 Mb (43 cM) to 121 Mb (61 cM). This locus interacts with Hdlq1 on chromosome 5. Homozygosity for 129S1/SvImJ-derived allele at both Hdlq1 and Hdlq16 significantly decreases plasma HDL concentration in male and female animals on CHOW diet.

References

• Original: J:144089 Su Z, et al., Genetic basis of HDL variation in 129/SvImJ and C57BL/6J mice: importance of testing candidate genes in targeted mutant mice. J Lipid Res. 2009 Jan;50(1):116-25
• All: 1 reference(s)