Heal28
QTL Variant Detail

Summary

• QTL variant: Heal28
• Name: wound healing/regeneration 28; SM/J
• MGI ID: MGI:5636707
• QTL: Heal28 Location: Chr9:60480714-60480860 bp Genetic Position: Chr9, Syntenic

Variant origin

• Strain of Specimen: SM/J

Variant description

• Allele Type: QTL

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:156191

LG/J mice, which display the same wound/regenerative healing phenotype as MRL/MpJ mice were crossed with the smaller SM/J mice. LG/J mice completely close wounds in their ear pinnae and SM/J do not. An F2 population of 357 progeny was scored for healing and genotypes were determined at 203 polymorphic markers. Composite interval mapping was then performed on the dataset to determine more precise QTL intervals.

Tests of significant linkage for a QTL were reported as a likelihood ratio test (LRT).

1000 permutations of the datasetgave the following estimates of QTL linkage:

LRT= 19.6 = highly significant;

LRT= 16.1 = significant; and

LRT= 14.5 = strongly suggestive.

Two highly significant peaks were identified on Chromosome 9, Heal28 and Heal29 respectively. Heal28 (chr9a) peaked at 60.0Mb with marker D9Mit207. Heal29 (chr9b) peaked between 85.0-98.0Mb with markers D9Mit12/rs13480313. The healing allele was derived from the LG/J parent for both QTL; neither QTL displayed sexual dimorphism.

03.04.2015 Curators Note: The authors suggest that these two QTL may be equivalent to Stheal8 and Stheal9, first mapped in 2001 (J:73197_Masinde). An (MRL/MpJ x SJL/J)F2 cross was used in that study, which differs from the cross used here, we consider this a separate map experiment and have named the QTL identified here, Heal28 and Heal29 respectively.

Two suggestively significant peaks, in female mice only, were observed on Chromosome 10. The stronger QTL of the two (chr10a) was labeled Heal16. It maps to 48.0Mb peaking with marker D10Mit170. The other, chr10b, mapped to approximately 85.0Mb peaking at marker rs8258353. Alleles were derived from the SM/J parent for both of these QTL.

Another highly significant marker, Heal26, mapped to Chromosome 11. This dimorphic marker mapped with significant linkage in males only between 83.0-92.0Mb peaking between markers D11Mit101-D11Mit122. The healing allele was derived from the LG/J parental strain.

03.04.2015 Curators Note: Heal26 is referred to in the map study asHeal10. Since Heal10 was orginally mapped in J:82708 in 2003 using (MRL/MpJ-Fas^lpr x C57BL/6J)F2 mice, which differs from the cross used here, we consider this a separate map study and have named the QTL Heal26.

A QTL, Heal27, mapped to chromosome 15.Significant linkage was seen at day 15 of healing. It mapped between 68.0-69.0Mb with peak markers rs13482628/D15Mit66. The allele was contributed from the SM/J.

03.03.2015 Curators Note: Author's note that this QTL co-localizes with Heal4. Since Heal4was originally mapped using an (MRL/MpJ-Fas^lpr x C57BL/6NTac)F2 intercross (J:50111) we consider the QTL mapped here, using a different cross, a novel QTL and have named it Heal27.

A suggestive QTL which the authors refer to as Heal15, mapped to Chromosome 2 with a peak between 0.0-3.8Mb.

1.20.2015 Curators Note: Heal15 was originally mapped in J:91685 in 2004 using an (MRL/MpJ x CAST/EiJ)F2 intercross. It appeared as only a suggestive marker in that cross also.

Haplotype asociation mapping analysis combined with global expression studies provides a list of candidate genes associated with healing and regeneration, see TABLE 4.

References

• Original: J:156191 Blankenhorn EP, et al., Genetic loci that regulate healing and regeneration in LG/J and SM/J mice. Mamm Genome. 2009 Nov-Dec;20(11-12):720-33
• All: 1 reference(s)