Oprk1^tm1.2Waca
Targeted Allele Detail

Summary

• Symbol: Oprk1^tm1.2Waca
• Name: opioid receptor, kappa 1; targeted mutation 1.2, William A Carlezon Jr
• MGI ID: MGI:5636742
• Synonyms: KOR^-
• Gene: Oprk1 Location: Chr1:5658689-5676354 bp, + strand Genetic Position: Chr1, 1.89 cM, cytoband A2-A3
• Alliance: Oprk1^tm1.2Waca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211750
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon3 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 3 via homologous recombination. Flp mediated recombination removed the neo cassette. Cre mediated recombination removed exon 3. Autoradiography revealed loss of functional protein, as reflected by binding of the highly selective OPRK1 agonist [3H]U69,593. Quantitative reverse transcription PCR (qPCR) analysis confirmed loss of mRNA expression throughout the brain of homozygous mutant mice. (J:211750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oprk1 Mutation: 30 strains or lines available

References

• Original: J:211750 Van't Veer A, et al., Ablation of kappa-opioid receptors from brain dopamine neurons has anxiolytic-like effects and enhances cocaine-induced plasticity. Neuropsychopharmacology. 2013 Jul;38(8):1585-97
• All: 2 reference(s)