Rnase9^tm1.1Xen
Targeted Allele Detail

Summary

• Symbol: Rnase9^tm1.1Xen
• Name: ribonuclease, RNase A family, 9 (non-active); targeted mutation 1.1, Taconic Biosciences
• MGI ID: MGI:5637811
• Synonyms: Rnase9^-, Rnase9^tm1Klm
• Gene: Rnase9 Location: Chr14:51275916-51279326 bp, - strand Genetic Position: Chr14, 26.35 cM, cytoband B-C1
• Alliance: Rnase9^tm1.1Xen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212760
• Parent Cell Line: XSV1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 2 via homologous recombination. Cre mediated recombination removed exon 2 and the neo cassette. Western blot analysis confirmed the absence of protein expression in epididymal extracts from sexually mature homozygous mutant mice. (J:212760)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rnase9 Mutation: 19 strains or lines available

References

• Original: J:212760 Westmuckett AD, et al., Impaired sperm maturation in RNASE9 knockout mice. Biol Reprod. 2014 Jun;90(6):120
• All: 1 reference(s)