Tg(Tcrb-TCF3/PBX1)23Gusa
Transgene Detail

Summary

• Symbol: Tg(Tcrb-TCF3/PBX1)23Gusa
• Name: transgene insertion 23, Guy Sauvageau
• MGI ID: MGI:5638104
• Synonyms: E2a-PBX1
• Transgene: Tg(Tcrb-TCF3/PBX1)23Gusa Location: unknown
• Alliance: Tg(Tcrb-TCF3/PBX1)23Gusa page

Transgene origin

• Strain of Origin: (C57BL/6J x C3H)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tcrb-TCF3/PBX1)23Gusa expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PBX1 (5087)

  Pbx1	(MGI:97495)

  human

  TCF3 (6929)

  Tcf3	(MGI:98510)

• Mutation details: The TCF3/PBX1 fusion oncogene (two transactivation domains of TCF3 fused to the C-terminal portion of PBX1) is under the control of lymphoid-specific promoter/enhancer elements in the pLIT3 expression vector, that include sequences from the proximal promoter of the Lck gene, immunoglobulin enhancer elements, and a TCR Vbeta promoter. The human growth hormone gene with a frameshift mutation in the coding region was inserted 3' of the transgene cDNA to provide introns that enhance the expression. Western blot analysis confirmed expression of the fusion protein in the bone marrow, spleen, and thymus. (J:95693)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:95693 Bijl J, et al., High incidence of proviral integrations in the Hoxa locus in a new model of E2a-PBX1-induced B-cell leukemia. Genes Dev. 2005 Jan 15;19(2):224-33
• All: 3 reference(s)