Magoh^{tm1d(KOMP)Dlsi}
Targeted Allele Detail

Summary

• Symbol: Magoh^{tm1d(KOMP)Dlsi}
• Name: mago homolog, exon junction complex core component; targeted mutation 1d, Debra L Silver
• MGI ID: MGI:5638556
• Synonyms: Magoh^del
• Gene: Magoh Location: Chr4:107736952-107744621 bp, + strand Genetic Position: Chr4, 50.18 cM
• Alliance: Magoh^{tm1d(KOMP)Dlsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213515
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 107737628 of Chromosome 4 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 107738438. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp mediated recombination. Subsequent cre mediated recombination created a null allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:213515)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Magoh Mutation: 16 strains or lines available

Notes

J:213515 used the KOMP vector PRPGS00072_A_G06 to create this allele.

References

• Original: J:213515 McMahon JJ, et al., Generation of a Magoh conditional allele in mice. Genesis. 2014 Aug;52(8):752-8
• All: 1 reference(s)