Coq8b^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Coq8b^{tm1b(EUCOMM)Hmgu}
• Name: coenzyme Q8B; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5638687
• Gene: Coq8b Location: Chr7:26932448-26957375 bp, + strand Genetic Position: Chr7, 15.85 cM, cytoband A3
• Alliance: Coq8b^{tm1b(EUCOMM)Hmgu} page
• IMPC: Coq8b gene page

Mutation origin

• Mutant Cell Line: HEPD0748_8_F07
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26938245 of Chromosome 7 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 26939943. Exons 5 and 6 are thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding this reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065, J:188991)

Expression

In Mice Carrying this Mutation:

35 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Coq8b Mutation: 30 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 4 reference(s)