Col1a1^tm3(Sry)Aqui
Targeted Allele Detail

Summary

• Symbol: Col1a1^tm3(Sry)Aqui
• Name: collagen, type I, alpha 1; targeted mutation 3, Alexander Quinn
• MGI ID: MGI:5638902
• Synonyms: Sry#18
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^tm3(Sry)Aqui page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215196
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Col1a1^tm3(Sry)Aqui expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sry (MGI:98660)

• Mutation details: RMCE on KH2 ES cells introduced into the 3' UTR the Sry coding region with 2095 bp of the upstream flanking inverted repeat and downstream inverted repeat up to but not including the StuI restriction site followed by an ampillicin resistance gene, PGK promoter, hygromycin resistance gene and polyadenylation sequence. FRT sites flank the entire insertion. (J:215196)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:215196 Quinn A, et al., A site-specific, single-copy transgenesis strategy to identify 5' regulatory sequences of the mouse testis-determining gene Sry. PLoS One. 2014;9(4):e94813
• All: 1 reference(s)