Igs2^{tm1(CAG-cas9*)Mmw}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(CAG-cas9*)Mmw}
• Name: intergenic site 2; targeted mutation 1, Monte Winslow
• MGI ID: MGI:5640626
• Synonyms: H11^LSL-Cas9
• Gene: Igs2 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(CAG-cas9*)Mmw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222466
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Endonuclease, No functional change)
• Mutation: Insertion
• Mutation details: The targeting construct contains a full-length attB site, the CMV enhancer/chicken beta-actin core promoter (CAGGS), a loxP-STOP(3xSV40 polyA)- loxP cassette (LSL), a 3xFLAG sequence, a nuclear localization signal (NLS), a human codon-optimized S. pyogenes cas9 gene (hSpCas9), a second NLS and the SV40 polyA signal. The embryonic stem cells used were previously targeted with the H11 allele ( Igs2^tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. One founder mouse carried the correctly targeted insertion of pattB-LSL-Cas9 into the first and second attP sites of the H11 locus. The resulting H11 knock-in allele is therefore attL-CAGGS-LSL-3xFLAG-NLS-hSpCas9-NLS-polyA-attR-attP-FRT5. (J:101977, J:222466)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

References

• Original: J:222466 Chiou SH, et al., Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015 Jul 15;29(14):1576-85
• All: 23 reference(s)