Picalm^tm1.2Tmae
Targeted Allele Detail

Summary

• Symbol: Picalm^tm1.2Tmae
• Name: phosphatidylinositol binding clathrin assembly protein; targeted mutation 1.2, Takahiro Maeda
• MGI ID: MGI:5640747
• Gene: Picalm Location: Chr7:89778711-89858655 bp, + strand Genetic Position: Chr7, 50.47 cM
• Alliance: Picalm^tm1.2Tmae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220728
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector contains an FRT-flanked PGK-neo cassette, in reverse orientation, and a 3' loxP site downstream of exon 2, and another loxP site inserted upstream of exon 2. Cre-mediated recombination removed the floxed exon 2 and neo cassette. No protein is detected by Western blot analysis (J:220728)

Expression

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Picalm Mutation: 42 strains or lines available

References

• Original: J:220728 Ishikawa Y, et al., Role of the clathrin adaptor PICALM in normal hematopoiesis and polycythemia vera pathophysiology. Haematologica. 2015 Apr;100(4):439-51
• All: 2 reference(s)