Col1a1^{tm2.1(CAG-cre/ERT2)Dgk}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2.1(CAG-cre/ERT2)Dgk}
• Name: collagen, type I, alpha 1; targeted mutation 2.1, David Kirsch
• MGI ID: MGI:5642281
• Synonyms: Col1a1^{FRT-Cre-ER-T2-FRT}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2.1(CAG-cre/ERT2)Dgk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Col1a1^{tm2.1(CAG-cre/ERT2)Dgk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: A targeting vector was designed to insert (from 5 to 3) a CAG promoter, a frt-flanked CreERT2 (Cre recombinase fused to an estrogen receptor ligand binding domain) coding sequence, and an attB/attP-flanked neomycin (neo) resistance cassette downstream of the collagen A1 (Col1a1) promoter. PhiC31-mediated recombination removed the neo cassette. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 3 reference(s)