Adgra1^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Adgra1^{tm1b(EUCOMM)Hmgu}
• Name: adhesion G protein-coupled receptor A1; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5642368
• Gene: Adgra1 Location: Chr7:139414090-139458004 bp, + strand Genetic Position: Chr7, 84.89 cM
• Alliance: Adgra1^{tm1b(EUCOMM)Hmgu} page
• IMPC: Adgra1 gene page

Mutation origin

• Mutant Cell Line: HEPD0764_3_E08
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 139431338 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 139432804. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding this knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065, J:188991)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Adgra1^tm1b(EUCOMM)Hmgu/Adgra1^tm1b(EUCOMM)Hmgu	B6N(Cg)-Adgra1^tm1b(EUCOMM)Hmgu/J	HEPD0764_3_E08

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms Sex:
Source:	IMPC - JAX	IMPC - JAX
homeostasis/metabolism	√
improved glucose tolerance	√
skeleton		√
increased bone mineral content		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adgra1 Mutation: 42 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 4 reference(s)