Med20em1(IMPC)J
Endonuclease-mediated Allele Detail
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Symbol: |
Med20em1(IMPC)J |
Name: |
mediator complex subunit 20; endonuclease-mediated mutation 1, Jackson |
MGI ID: |
MGI:5643678 |
Synonyms: |
Med20-, Med20em1J |
Gene: |
Med20 Location: Chr17:47922510-47935176 bp, + strand Genetic Position: Chr17, 23.66 cM
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Alliance: |
Med20em1(IMPC)J page
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IMPC: |
Med20 gene page |
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Med20em1(IMPC)J/Med20em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E3.5 but not at E7.5. Blastocysts grown in vitro fail to hatch from the zona pellucida.
Show the 1 phenotype image(s) involving this allele.
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Strain of Origin: |
C57BL/6NJ
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Project Collection: |
IMPC
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Allele Type: |
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Endonuclease-mediated (Null/knockout) |
Mutation: |
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Intragenic deletion
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Mutation details: This allele from project Med20-6705J-M5886 was generated at The Jackson Laboratory by injecting Cas9 RNA and 2 guide sequences, AGGAACTCTTGGGGACTGAT and GCTTAGAGTATTTACGTTAA (along with a plasmid containing 1 kb homology arms flanking the floxed critical exon, which did not integrate) which resulted in a 348bp deletion beginning in intron 1 at GGGGACTGATGGGTGGGGAT at Chromosome 17 positive strand position 47,612,827 bp (GRCm38) and ending after GCTTAGAGTATTTACGTTAAT at position 47,613,174 bp in intron 2. This mutation deletes exon 2 and is predicted to cause amino acid sequence changes after residue 4 and early truncation 11 amino acids later. PCR failed to detect the insertion of any loxP sites. RT-PCR analysis confirmed the absence of mRNA expression in homozygous mutant blastocysts.
(J:188991, J:286962)
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Inheritance: |
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Not Specified |
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
5 reference(s) |
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