Pdgfra^{tm1.1(EGFP/cre/ERT2)Hyma}
Targeted Allele Detail

Summary

• Symbol: Pdgfra^{tm1.1(EGFP/cre/ERT2)Hyma}
• Name: platelet derived growth factor receptor, alpha polypeptide; targeted mutation 1.1, Hiroyuki Miwa
• MGI ID: MGI:5644346
• Synonyms: PDGFRalpha^GCE
• Gene: Pdgfra Location: Chr5:75312953-75358876 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Pdgfra^{tm1.1(EGFP/cre/ERT2)Hyma} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221424
• Parent Cell Line: KTPU8 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Pdgfra^{tm1.1(EGFP/cre/ERT2)Hyma} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pdgfra (MGI:97530)

• Mutation details: A fusion of EGFP and an inducible cre recombinase gene (EGFP/cre/ERT2) was inserted at the translation initiation site. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the fusion. (J:221424)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdgfra (mouse) Summary of all recombinase alleles driven by Pdgfra. Comparative matrix view of recombinase activities Alliance mouse Pdgfra gene page

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdgfra Mutation: 88 strains or lines available

References

• Original: J:221424 Miwa H, et al., Generation and characterization of PDGFRalpha-GFPCreER(t2) knock-In mouse line. Genesis. 2015 May;53(5):329-36
• All: 7 reference(s)