Faah^tm1.1Flee
Targeted Allele Detail

Summary

• Symbol: Faah^tm1.1Flee
• Name: fatty acid amide hydrolase; targeted mutation 1.1, Francis S Lee
• MGI ID: MGI:5644434
• Synonyms: FAAH C385A
• Gene: Faah Location: Chr4:115853866-115876034 bp, - strand Genetic Position: Chr4, 53.08 cM
• Alliance: Faah^tm1.1Flee page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221783
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

Allele Type: Targeted (Humanized sequence) Mutation: Nucleotide substitutions Mutation details: A C to A transversion was introduced at coding nucleotide 385 (c.385C>A) in exon 3. The FRT site flanked neomycin resistance gene cassette that was inserted was subsequently removed through flp-mediated recombination. The mutation results in the substitution of a conserved proline at amino acid position 129 with a threonine residue (p.P129T). This mimics a polymorphism found in humans where it renders the enzyme susceptible to increased proteolytic degradation. Western blot analysis revealed an allele dose-dependent decrease in protein expression levels in forebrain homogenates from heterozygous and homozygous mutant mice relative to wild-type controls. Hydrolytic activity was reduced while anandamide (AEA) levels were increased in mutant brain homogenates relative to wild-type controls. (J:221783)

Generation of the Faah^tm1.1Flee alelle

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Faah Mutation: 28 strains or lines available

References

• Original: J:221783 Dincheva I, et al., FAAH genetic variation enhances fronto-amygdala function in mouse and human. Nat Commun. 2015;6:6395
• All: 4 reference(s)