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Igs7tm85.1(tetO-gltI/GFP*)Hze
Targeted Allele Detail
Summary
Symbol: Igs7tm85.1(tetO-gltI/GFP*)Hze
Name: intergenic site 7; targeted mutation 85.1, Hongkui Zeng
MGI ID: MGI:5645698
Synonyms: Ai85(TITL-iGluSnFr), Igs7
Gene: Igs7  Location: unknown  Genetic Position: Chr9, Syntenic
Alliance: Igs7tm85.1(tetO-gltI/GFP*)Hze page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:219930
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Inducible, Reporter)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Mutation detailsThe pTRE-LSL-iGluSnFr replacement vector was designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element, a modified Tet response element promoter, a floxed-STOP cassette, the iGluSnFr coding sequence (details below), a WPRE sequence (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. The iGluSnFr fusion gene is constructed from the Escherichia coli gltI gene and circularly permutated green fluorescent protein (cpGFP). Specifically, iGluSnFr has a 21 aa IgG K-leader sequence, the gltI aa 1-253, the LILV linker, the cpGFP (aa 148-239, an 18 bp flexible linker and aa 1-147), the L2NP linker, the gltI aa 254-279, an 11 aa c-Myc epitope and a 49 aa PDGR sequence (PDGR aa 513-561). iGluSnFr is an extremely fast and sensitive glutamate indicator with large signal-to-noise ratio, and kinetics appropriate for in vivo imaging. PhiC31-mediated recombination removed the AttB/AttP-flanked PGK-hygromycin-SV40polyA cassette and replaced it with the recombined AttB/AttP site (AttL). (J:219930)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igs7 Mutation:  66 strains or lines available
References
Original:  J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory