Igs7tm92.1(tetO-ECFP*/Venus*)Hze
Targeted Allele Detail
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Symbol: |
Igs7tm92.1(tetO-ECFP*/Venus*)Hze |
Name: |
intergenic site 7; targeted mutation 92.1, Hongkui Zeng |
MGI ID: |
MGI:5645699 |
Synonyms: |
Ai92(TITL-YCX2.60), Igs7tm92.1(tetO-YCX2,60)Hze, Igs7tm92.1(tetO-YCX2.60)Hze |
Gene: |
Igs7 Location: unknown Genetic Position: Chr9, Syntenic
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Alliance: |
Igs7tm92.1(tetO-ECFP*/Venus*)Hze page
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Allele Type: |
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Targeted (Conditional ready, Inducible, Reporter) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Mutation details: The vector was designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element, a modified Tet response element promoter, a floxed-STOP cassette, the YCX2.60 coding sequence [an enhanced cyan fluorescent protein with C-terminal 11 amino acids deleted (ECFPDeltaC11 with R27K and K213N mutations, a rat calmodulin DNA fragment (CaM; aa 5-148 with N60D and D78Y mutations [to increase the fluorescence change for small calcium transients] and E127V mutation), a GG linker, a chicken smooth muscle M13 fragment of myosin light chain kinase (with I-to-S mutation in the 20th aa of the sequence), and a circularly permutated Venus enhanced yellow fluorescent protein (cp173 Venus; aa 174-239, a 15 bp linker and aa 1-173)], a WPRE sequence, a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site.
The fluorescence resonance energy transfer (FRET)-based calcium sensor Yellow Cameleon variant X 2.60 (YCX2.60) is an improved version of YC2.60 with expanded dynamic range and 2-3-fold greater dissociation constant. The fusion gene is an enhanced cyan fluorescent protein with C-terminal 11 amino acids deleted, a rat calmodulin DNA fragment (CaM; aa 5-148 with N60D and D78Y mutations [to increase the fluorescence change for small calcium transients] and E127V mutation), a GG linker, a chicken smooth muscle M13 fragment of myosin light chain kinase (with I-to-S mutation in the 20th aa of the sequence), and a circularly permutated Venus enhanced yellow fluorescent protein (cp173 Venus; aa 174-239, a 15 bp linker and aa 1-173). PhiC31-mediated recombination removed the AttB/AttP-flanked PGK-hygromycin-SV40polyA cassette and replaced it with the recombined AttB/AttP site (AttL).
(J:219930)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58 |
All: |
2 reference(s) |
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